Grey Willows growing around the pond dominate the site, with a few other mature trees such as Ash, Pedunculate Oak and Sycamore. There is an extensive understory of Hawthorn and Blackthorn with Hazel, Field Maple and Crab Apple. Brambles, Dog Rose, Honeysuckle and Ivy create dense areas of sheltered habitat as well as attracting butterflies, moths and bees.

The damp and shady conditions encourage the growth of Male Ferns, mosses, lichens and fungi. Flowering plants of shady woodland include Stinking Iris (May-July), Wood Avens (May-November) and Clustered Dock (June-October), while Common Centaury (June-October) grows in the slightly sunnier glades.

On the car parking area and former bramble patch the flora is typical of areas of disturbed or waste ground, including Bristly Oxtongue (June-November), Broad-leaved Dock (June-October), Canadian Fleabane (July to September), Common Field Speedwell (all year), Common Mouse-ear (April-September), Common Ragwort (June-November), Creeping Thistle (June-October), Field Forget-me-not (April to October), Great Mullein (June-August), Greater Plantain (all year),  Lesser Burdock (July-September), Nipplewort (June-October), Prickly Sowthistle (June-August), Redshank (June-October), Spear Thistle (July-October), and Stinging Nettle.

Among the mammals using the site are Common and Water Shrew (the UK’s only venomous mammal) and Wood Mouse. Seven species of bat have been identified at Wood Norton, including the rare Barbastelle, as well as Daubenton's, Serotine, Noctule, Common and Soprano Pipistrelle and Brown Long-eared Bat.

Spring-time brings the sound of summer migrants such as Blackcap, Chiffchaff and Whitethroat, as well as resident species including Bullfinch, Chaffinch, Greenfinch, Linnet, Marsh Tit, Siskin, Song Thrush and Wren. The pond attracts ducks including Gadwall and Mallard as well as Grey Heron, Grey Wagtail, Moorhen and Snipe. Meanwhile, the woodland provides homes for Great Spotted Woodpecker, Green Woodpecker, Stock Dove and Tawny Owl. In winter, species such as Woodcock and Redwing use the site for shelter and feeding.