This site features some interesting flowering plants, including Black Horehound( June-September), Bluebell (April-May), Common Mallow (June-October) and Musk-mallow (July-August), Cut-leaved Crane's-bill (May-August.), Dog's Mercury – an indicator of ancient woodland, Garlic Mustard (April- July), Ground Ivy (January to December – an excellent source of early nectar and pollen for bees),  Rough Chervil (May-July), Short-fruited Willowherb (July-August), - Ransoms, otherwise known as Wild Garlic (April-June), White Bryony (May-September), Wood Avens (May to August), and Stinking Iris (May-July) – whose crushed leaves smell like beef crisps!

There are some unusual trees and shrubs at this location, including Bullace, Buckthorn, Cherry, Goat Willow, Grey Willow, Lime and Spindle. Coppiced Hazel is a feature of the site.

A number of tit species frequent Brinton Nine Holes. Including Marsh Tit and Coal Tit as well as the commoner Blue, Great and Long-tailed varieties. Both Great-spotted and Green Woodpeckers have been seen at the site, while Treecreepers can be seen running up the trunks of the trees. Spring brings the sound of singing Blackcaps and Chiffchaffs, while autumn and winter draw in Woodcock and Redwings.

The grassland and woodland edges attract Holly Blue (April-May and August), Large White (April-October), Red Admiral (June-September) and Speckled Wood (April-September) butterflies, as well as bees such as the White-tailed Bumblebee. As the grassland and wildflower meadow develops, more species of butterflies, bees and other insects should start to appear.

Several species of dragonfly have put in an appearance at the site, including Common Darter (July-October) and the aptly named Willow Emerald Damselfly (May to August).